INTRODUCTION: - First, S. aureus infections caused by penicillin-resistant S. aureus were treated with methicillin in 1959. The United Kingdom reported that S. aureus isolates had developed methicillin resistance (Methicillin-Resistant S. aureus) and numerous MRSA isolates were subsequently reported from other European nations as well as Japan, Australia, and the United States. Methicillin-resistant S. aureus (MRSA) is known to be resistant to several different antibiotic classes. AIM:-To compare conventional methods for MRSA detection with the Molecular approach of detecting mecAgene through PCR for accuracy. MATERIALS AND METHODS: -This study was conducted in the Department of Microbiology IMCH&RC, Indore for 18 months. Clinical samples from OPD/IPD were collected and processed by standard procedures and AST was performed according to the CLSI 2024. The MRSA species were examined for Cefoxitin susceptibility by the Disc Diffusion (DD) method and E test for the detection of Minimal Inhibitory Concentration (MIC). The drug-resistant gene mec-A was detected by conventional PCR. RESULT: - Out of 110 isolates, 45 isolates of MRSA were found to be Cefoxitin Resistance by Disc Diffusion (Kirby Baur’s) Method. The Prevalence of MRSA was found by DD method for the detection of 45/110 (40.91%) and E test by 40/110 (36.3%) in the present study. The molecular characterization confirms 36/40 MRSA for the mecA gene. CONCLUSION:- The prevalence MRSA for the mec A gene among was observed to be 32.7%%. Prolonged hospital stay and direct antibiotic pressure in the hospital can lead to the development of MRSA variants.