Background: Catheter-associated infections (CAIs), especially catheter-associated urinary tract infections (CAUTI), are major causes of hospital-acquired infections. Biofilm formation on catheter surfaces is a key mechanism, leading to antibiotic resistance and treatment failure.

Aim: To evaluate the prevalence of biofilm formation in catheter-associated infections and to assess antibiotic sensitivity patterns of biofilm-producing organisms.

Materials and Methods: A cross-sectional study was conducted on 150 catheterized patients. Urine samples and catheter tips were collected for culture, biofilm detection (tube method & Congo red agar), and antibiotic sensitivity testing.

Results: Out of 150 samples, 95 (63.3%) showed bacterial growth. Among them, 67 (70.5%) were biofilm producers. The most common organism was E. coli (45%), followed by Klebsiella spp. (25%) and Pseudomonas spp. (15%). Biofilm-producing strains showed high resistance to cephalosporins and fluoroquinolones, but good sensitivity to carbapenems and colistin.

Conclusion: Biofilm formation is strongly associated with catheter-associated infections and leads to multidrug-resistant pathogens. Regular catheter surveillance, antibiotic stewardship, and use of coated catheters are recommended.