Background: Urinary tract infection (UTI) is one of the most common infections worldwide. The emergence of Extended-Spectrum Beta-Lactamase (ESBL) producing organisms has become a major challenge due to high resistance to many antibiotics.

Aim: To determine the prevalence of ESBL-producing organisms in urine samples and study their antibiotic sensitivity patterns.

Materials & Methods: A cross-sectional study was conducted on 200 urine samples from suspected UTI patients. Culture, identification, and ESBL detection were performed using the Double Disk Synergy Test (DDST) and phenotypic confirmatory disc diffusion test (PCDDT). Antibiotic susceptibility was tested using Kirby–Bauer method.

Results: Out of 200 samples, 98 (49%) showed significant bacterial growth. Among them, 54 (55.1%) were confirmed ESBL-producers. E. coli (60%) was the most common ESBL-producing organism, followed by Klebsiella spp. (25%). ESBL strains showed high resistance to cephalosporins and fluoroquinolones but good sensitivity to carbapenems and piperacillin-tazobactam.

Conclusion: A high prevalence of ESBL-producing organisms was observed in urine samples, indicating the need for routine ESBL detection and antibiotic stewardship to prevent treatment failure.